CCR4/Gα15/CHO
CBP71336
| I. Background | |
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CCR4（又稱 CD194、CMKBR4）是編碼 CC 趨化因子受體 4 的蛋白編碼基因，屬于 G 蛋白偶聯(lián)受體家族的關(guān)鍵成員。該基因主要在 Th2 細(xì)胞、調(diào)節(jié)性 T 細(xì)胞（Tregs）等 T 細(xì)胞亞群中選擇性表達(dá)，在 B 細(xì)胞、NK 細(xì)胞、巨噬細(xì)胞中也有少量表達(dá)，同時在成人 T 細(xì)胞 白血病/淋巴瘤（ATLL）等多種腫瘤細(xì)胞中存在高表達(dá)。其編碼的受體與 CCL17、CCL22 等配體結(jié)合后，可激活 ERK、MAPK、PI3K-AKT 等下游通路，調(diào)控免疫細(xì)胞定向遷移、細(xì)胞趨化與活化，參與免疫穩(wěn)態(tài)維持、炎癥反應(yīng)等生理過程。 |
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| II. Description | |
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CCR4 Gα15 CHO 細(xì)胞藥靶模型很好的模擬了體內(nèi) CCR4 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
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| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
F12K+10%FBS+100μg/ml Hygromycin B+200μg/ml G418 |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CCR4 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 2. Dose Response of Recombinant Human CCL22 in CCR4 Gα15 CHO(C11). |
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