CD19 KO Luc Raji
CBP74549
詢 價(jià)
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產(chǎn)品描述
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| I. Background | |
| CD19(Cluster of Differentiation 19)是一種跨膜糖蛋白,屬于免疫球蛋白超家族成 員,主要表達(dá)于 B 淋巴細(xì)胞譜系細(xì)胞表面,包括前 B 細(xì)胞、成熟 B 細(xì)胞及大多數(shù) B 細(xì)胞惡 性腫瘤細(xì)胞。其功能是作為 B 細(xì)胞受體(BCR)復(fù)合物的共受體,參與 B 細(xì)胞活化、增殖 和分化信號(hào)的傳導(dǎo)。CD19 作為 B 細(xì)胞惡性腫瘤的核心靶點(diǎn),推動(dòng)了 CAR-T 等精準(zhǔn)免疫治 療的發(fā)展,顯著改善了患者預(yù)后。 | |
| II. Description | |
| CD19 KO Luc Raji 細(xì)胞,在 Raji 細(xì)胞的基礎(chǔ)上,穩(wěn)定敲除人 CD19。 | |
| III. Introduction | |
| 敲除基因: | CD19 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS +1 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Representative Data | |
![]() Figure 1. CD19 KO Luc Raji 細(xì)胞,在 Raji 細(xì)胞的基礎(chǔ)上,穩(wěn)定敲除人 CD19。. ![]() Figure 2. CD19 KO Luc Raji 測序驗(yàn)證結(jié)果, hg38:chr16-28933026-TG-(CD19:p.W159Gfs*6) CD19(NM_001770.6): c.475_476del TG. ![]() Figure 3. CD19 KO Luc Raji 細(xì)胞 Luc 驗(yàn)證結(jié)果。 Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). |
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